biology
Why are antibiotics and vitamins not normally autoclaved when preparing microbiological media?
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Antibiotics and vitamins are not normally autoclaved when preparing microbiological media because the heat of the autoclaving process can denature or destroy them. Autoclaving is a sterilization process, not a means of preserving the compounds. The efficacy of the antibiotics and vitamins can decrease significantly if autoclaved and would not provide suitable nutrients for the growth of the desired microorganisms.
